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Discover NAD3®: The Longevity Ingredient Beyond Boosting NAD+

April 26, 2024 7 min read

Discover NAD3®: The Longevity Ingredient Beyond Boosting NAD+

Humans' eternal chase of longevity and vitality has been at the forefront of study and science for as long as human history itself. Today, the study of molecular pathways of aging opens up before us, and there are interventions that promise to reduce the advancement of aging.

At the center of this study is one molecule: nicotinamide adenine dinucleotide (NAD+), a coenzyme critical for human cellular function and health. In this comprehensive look, we will discover what NAD+ is, explore the key importance sirtuins and PARPs play, discuss ways through which we can keep naturally high NAD+ levels, and look at how NAD3® is revolutionizing the industry with the potential to extend your health span.

NAD3® is a unique ingredient used in only a few specialist supplement formulations, including NAD Regen by BioStack. We'll get in to more detail of NAD3® shortly but first...

Understanding NAD+ and Its Role in Longevity

NAD+ is a coenzyme found in every living cell of a very common nature and is required for most sets of biological processes important for cellular function and health. NAD+ is an obligatory substrate for the sirtuin family of proteins and poly(ADP-ribose) polymerases (PARPs), implicated in the regulation of processes related to critical metabolism, DNA repair, and stress responses. Latest studies have observed NAD+ as the coenzyme required for electron transfer and helping in energy metabolism within cells through redox reactions.

The Importance of NAD+ for Longevity

It is essential to maintain the required NAD+ level for the continuation of cell health and its invigorating resilience as we age. Physiological studies have exhibited a clear downfall of NAD+ levels that correlate directly with age-associated decline and may contribute to age-related conditions such as metabolic dysfunction, neurodegeneration, and cardiovascular disorders.

Read more about the physiological impact of NAD+

Bolstering NAD+ levels through supplementation and other lifestyle adaptations may potentially dull the decline NAD+ is associated with age and support health in various ways, including:

  • Activating sirtuins: Sirtuins are a class of NAD+-dependent longevity factors. Sirtuins respond to adequate NAD+ supplies and could therefore be one explanation for an increase in cellular repair mechanisms, manifesting, for example, in greater resistance to stress and, potentially, longevity. NAD+ is also involved in the modulation of other vital chromatin activities in the nucleus, including DNA repair and gene regulation.

  • Activation of PARPs: Poly (ADP-ribose) polymerase (PARPs) is an enzyme mainly included in the repair of DNA. NAD+ activated PARP in the course of DNA repair and maintenance, hence keeping the stability and integrity of the genome. In addition, it turns out that PARPs play a role in other cellular functions and have been implicated in the regulation of apoptotic processes and inflammation.

  • Regulation of Metabolic Processes: Regulation of NAD+ is involved in an intricate regulation of various metabolic pathways, such as glucose and lipid metabolism. Extremely importantly, the sirtuins take part in the regulation of metabolism by affecting processes that include gluconeogenesis, fatty acid oxidation, and insulin sensitivity.

Natural Methods to Boost NAD Levels

In addition to supplementation, several lifestyle and dietary choices may impact NAD+ levels naturally in a way that is humanly possible.

  • Caloric Restriction: Studies have shown that caloric restriction increases the NAD+ level to promote sirtuins for longevity and healthspan. Caloric restriction in animal studies without malnutrition has been associated with a long list of health improvements, from metabolic health to longer life.

  • Intermittent Fasting: This study showed increased NAD+ levels in the body and enhanced cellular resilience achieved through alternating cycles of fasting and feeding. Fasting may produce cellular responses that upregulate the levels of autophagy, DNA repair, and resistance to stress, which are all regulated by NAD+ levels.

  • Regular Physical Activity: Regular physical activity is also linked with the increased levels of NAD+ and activity of sirtuins in support of healthy anti-aging cellular function and metabolism. Basically, exercise enhances mitochondrial biogenesis, all antioxidant defenses, and the metabolic health of all the features that are influenced by the control of intracellular NAD+ availability.

  • Healthy Diet: A diet containing NAD precursors, including niacin (vitamin B3), tryptophan, and nicotinamide riboside (NR), is helpful in NAD+ synthesis for cell improvement. Foods are rich in niacin and its precursor tryptophan, while nicotinamide riboside is contained at low levels in milk and other dairy products.

NAD3®: A True Breakthrough in NAD+ Supplementation

Amidst a sea of NAD+ boosting supplements on the market, NAD3® exemplifies a game-changer in the approach to NAD+ status with its unique advantage over classic NAD+ precursors. Unlike nicotinamide riboside (NR) and nicotinamide mononucleotide (NMN), which may actually be burdensome to the methylation pathways, and upregulate inflammation, NAD3® presents a much safer, effective strategy to optimize NAD+ levels without compromise to methylation.

The magic comes in because NAD3® has a synergetic mix of three primary ingredients:

  • Cuprous Niacin: Niacin or vitamin B3 is an important precursor of NAD, where it acts as a cofactor to a series of mitochondrial reactions related to energy metabolism. Niacinamide, a form of niacin, is included in the NAD Regen formula to enhance its efficacy. Niacin is converted into NAD+ through the Preiss-Handler pathway, contributing directly to NAD+ synthesis.

  • Theacrine: Theacrine, a compound often compared to caffeine but devoid of its negative side effects, possesses various health benefits, including inflammation reduction. Given that chronic low-level inflammation is implicated in NAD+ depletion in aging individuals, the inflammation-modulating properties of theacrine are particularly relevant for preserving NAD+ levels. Theacrine is believed to enhance NAD+ levels indirectly by reducing oxidative stress and inflammation, thereby preserving NAD+ availability for other cellular processes.

  • Wasabi Japonica (with 6-MSITC): This bioactive compound derived from wasabi japonica has been shown to upregulate SIRT1 protein levels, a key player in cellular metabolism and longevity. By enhancing SIRT1 activity, wasabi japonica complements the effects of niacin and theacrine in promoting NAD+ production and recycling. Wasabi japonica contains 6-MSITC, a compound that activates SIRT1 indirectly by inhibiting certain signaling pathways involved in its degradation.

NAD3® doesn't work like most traditional NAD-supporting supplements that only use precursor supplementation of NAD+. NAD3® targets this central problem to the root of NAD decline through direct influence over the Salvage Pathway. The product's recycling aspect conundrum brings to light the age-related decline of NAD+ levels, a problem that NAD3® squarely meets with its synergistic composition of Niacinamide, Theacrine, and Wasabi Japonica.

Let's take a look at the first human trial done on NAD3

Enhance Trial: Effects of NAD3® on Hallmarks of Aging and Clinical Endpoints of Health in Middle Aged Adults: A Subset Analysis Focused on Blood Cell NAD+ Concentrations and Lipid Metabolism

The study titled "Enhance Trial: Effects of NAD3® on Hallmarks of Aging and Clinical Endpoints of Health in Middle Aged Adults: A Subset Analysis Focused on Blood Cell NAD+ Concentrations and Lipid Metabolism" by Michael D. Roberts et al. investigated the effects of a theacrine-based supplement, NAD3®, on lipid metabolism and cellular NAD+ status in middle-aged adults.

The study involved 28 participants who were randomly assigned to receive either NAD3 or a placebo for 12 weeks. Blood samples were collected before and after the intervention to assess serum lipids and NAD+ metabolites in peripheral blood mononuclear cells (PBMCs).

The results showed significant decreases in total cholesterol, LDL cholesterol, and LDL: HDL ratio in the NAD3 group compared to the placebo group. Additionally, PBMC NAD+: NADH values increased significantly in the NAD3 group compared to the placebo group.

No significant changes were observed in other clinical markers. The study suggests that NAD3 supplementation may have beneficial effects on lipid metabolism and cellular NAD+ status in middle-aged adults, indicating its potential role in promoting healthy aging.

A Theacrine-Based Supplement Increases Cellular NAD+ Levels and Affects Biomarkers Related to Sirtuin Activity in C2C12 Muscle Cells In Vitro

This study investigated the effects on sirtuin activity and mitochondrial biogenesis markers in skeletal muscle cells in vitro. The researchers used the murine C2C12 myoblast cell line for their experiments. NAD3 treatment increased Sirt1 mRNA levels and global sirtuin activity compared to control-treated cells.

Additionally, NAD3 treatment increased NAMPT and SIRT1 protein levels and cellular NAD+ concentrations. However, markers of mitochondrial biogenesis were minimally affected by NAD3 treatment.

The findings suggest that NAD3 supplementation may modulate select biomarkers associated with NAD+ biogenesis and sirtuin activity.

The third and most recent study in 2023 titled ”The Effects of a Multi-Ingredient Supplement Containing Wasabia Japonica Extract, Theacrine, and Copper (I) Niacin Chelate on Peripheral Blood Mononuclear Cell DNA Methylation, Transcriptomics, and Sirtuin Activity”

The Effects of a Multi-Ingredient Supplement Containing Wasabia Japonica Extract, Theacrine, and Copper (I) Niacin Chelate on Peripheral Blood Mononuclear Cell DNA Methylation, Transcriptomics, and Sirtuin Activity

This study looked at the effects of NAD3 on peripheral blood mononuclear cell (PBMC) DNA methylation, transcriptomics, and sirtuin activity in middle-aged adults after 12 weeks of supplementation.

Key findings include: Transcriptomic analysis revealed significant alterations in PBMC mRNA expression between the supplement and control groups, with several biological processes predicted to be affected, such as DNA conformational changes and DNA replication.

DNA methylation analysis showed differences in methylation changes between the groups, with the supplement group exhibiting fewer hypermethylated CpG sites compared to the control group. PBMC sirtuin activity was maintained in the supplement group but decreased in the control group over the 12-week period, suggesting a potential role of the supplement in maintaining sirtuin activity through the maintenance of NAD+: NADH levels.

Telomere length showed a decreasing trend in both groups, but the supplement group exhibited a less pronounced decrease compared to the control group. These findings suggest that the multi-ingredient supplement affects cellular processes related to gene expression, DNA methylation, and sirtuin activity in middle-aged adults. However, like any other study further research is needed to elucidate the specific mechanisms and clinical implications of these effects.


NAD+ is literally at the core of the cutting edge of longevity and optimal health, deeply holding implications for aging and age-associated diseases. This would suggest that people, both men and women, who use formulations like NAD3® could be giving themselves the ability to support healthy cellular function and increase longevity.

Indeed, increased research and advancement in NAD+ science can only mean an even brighter future ahead in relation to harnessing the powers of NAD+ to unlock the secrets of longevity and change the landscape of aging.